LOCUS       40924_31800       12892 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB404 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12892)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 12892)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12892)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    334..458
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        495..525
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             579..1235
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1580..1882
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1926..2050)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2085..3893
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3967..4219
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4253..4269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4277..4293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4301..4331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4346..4367)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4452..4626)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4686..5474)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5543..6219)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(6414..6438)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6959..7747
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7996..8584
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8770..8910)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9254..9448)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9517..10587)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11019..11645)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaat caaacaagtt tgtacaaaaa agctgaacga
      361 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      421 acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga ggggccgcat
      481 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
      541 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      601 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      661 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      721 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      781 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
      841 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
      901 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
      961 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1021 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1081 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1141 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1201 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
     1261 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     1321 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     1381 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     1441 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     1501 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     1561 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     1621 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     1681 ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc cgtgaacttt acccggtggt
     1741 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     1801 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     1861 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     1921 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     1981 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     2041 acaaagtggt tcgatctaga ggatccccgg gtggtcagtc ccttatgtta cgtcctgtag
     2101 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
     2161 aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg
     2221 ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgcgggca
     2281 acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
     2341 tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg
     2401 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
     2461 aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg
     2521 gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
     2581 actatgccgg aatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
     2641 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
     2701 tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
     2761 ctggacaagg cactagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
     2821 aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc
     2881 cgcttcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca
     2941 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat
     3001 tcgataacgt gctgatggtg cacgaccacg cattaatgga ctggattggg gccaactcct
     3061 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
     3121 tcgtggtgat tgatgaaact gctgctgtcg gctttaacct ctctttaggc attggtttcg
     3181 aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc agtcaacggg gaaactcagc
     3241 aagcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg
     3301 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
     3361 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg
     3421 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
     3481 accgttatta cggatggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
     3541 aaaaagaact tctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
     3601 gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc
     3661 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
     3721 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
     3781 acaagaaagg gatcttcact cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa
     3841 aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa caatgaatca
     3901 acaactctcc tggcgcacca tcgtcggcta cagcctcggg aattgctacc gagctcgaat
     3961 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     4021 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     4081 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     4141 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     4201 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     4261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     4381 gcgcctattt ctgaattcaa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     4441 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
     4501 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     4561 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
     4621 gagaaactcg agcttgtcga tcgactctag ctagaggatc gatccgaacc ccagagtccc
     4681 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     4741 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     4801 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     4861 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgtgtc
     4921 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
     4981 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     5041 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     5101 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     5161 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     5221 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     5281 cgcgctgcct cgtcctggag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     5341 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     5401 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     5461 ccatcttgtt caatccccat ggtcgatcga cagatctgcg aaagctcgag agagatagat
     5521 ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata
     5581 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
     5641 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     5701 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
     5761 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
     5821 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
     5881 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
     5941 agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt
     6001 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     6061 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     6121 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     6181 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     6241 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc
     6301 tgctctagcc aatacgcaaa ccgaattcag aaataaattc agcctaattc ggcgttaatt
     6361 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     6421 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     6481 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     6541 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     6601 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     6661 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     6721 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     6781 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     6841 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     6901 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     6961 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     7021 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     7081 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     7141 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     7201 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     7261 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     7321 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     7381 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     7441 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     7501 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     7561 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     7621 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     7681 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     7741 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     7801 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     7861 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     7921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8101 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     8161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8761 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     8821 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     8881 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     8941 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     9001 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     9061 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     9121 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     9181 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     9241 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     9301 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     9361 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     9421 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     9481 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     9541 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     9601 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     9661 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     9721 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     9781 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     9841 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     9901 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     9961 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
    10021 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
    10081 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
    10141 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
    10201 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
    10261 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
    10321 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
    10381 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
    10441 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
    10501 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
    10561 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
    10621 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
    10681 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
    10741 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
    10801 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
    10861 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
    10921 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
    10981 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
    11041 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    11101 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    11161 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    11221 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    11281 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    11341 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    11401 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    11461 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    11521 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    11581 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    11641 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    11701 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    11761 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    11821 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    11881 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    11941 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    12001 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    12061 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    12121 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    12181 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    12241 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    12301 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    12361 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    12421 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    12481 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    12541 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    12601 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    12661 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    12721 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    12781 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
    12841 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac ct
//
